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The Alu I-induced bands in metaphase chromosomes of orangutan (Pongo pygmaeus)

โœ Scribed by G. F. Stefano; E. Romano; L. Ferrucci

Publisher
Springer
Year
1986
Tongue
English
Weight
596 KB
Volume
72
Category
Article
ISSN
0340-6717

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โœฆ Synopsis

Restriction endonucleases have been recently proved to be active on fixed chromosomes, thus they are useful in chromatin structure studies. Within this class of enzymes, Alu I is able to detect the presence and localization of highly repetitive DNA sequences in human and in other mammalian and dipteran species. In this paper the pattern obtained on fixed metaphase chromosomes of orangutan (Pongo pygmaeus) by Alu I digestion and Giemsa staining is shown. The results are discussed in the light of the distribution, in this species, of the I-IV human satellite DNAs. It is also suggested that in Pongo some highly repetitive sequences, different from the major human satellites, are present.

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