The alternate expression of two restriction and modification systems

✍ Scribed by Glover, S. W. ;Firman, K. ;Watson, G. ;Price, C. ;Donaldson, S.

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 846 KB
• Volume: 190
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00330325

✦ Synopsis

Plasmids R124 and R124/3 carry genes coding for two different R-M systems and normally only one set of genes is expressed. These genes can be translocated to F plasmids that are compatible with the R factors and in strains carrying these F plasmids and an R factor a transacting regulatory mechanism switches off the expression of R-M genes on the introduced plasmid. Additionally the unexpressed genes on the introduced plasmid are expressed. The regulatory mechanism controlling the alternative expression of R124 and R124/3 R-M genes involves a physical rearrangement of DNA sequences.