The affinity of syncytium, fibrin and fibrinoid of the human placenta for acid and basic dyes under controlled conditions of staining

✍ Scribed by Singer, Marcus ;Wislocki, George B.

• Book ID: 102742926
• Publisher: John Wiley and Sons
• Year: 1948
• Tongue: English
• Weight: 922 KB
• Volume: 102
• Category: Article
• ISSN: 0003-276X
• DOI: 10.1002/ar.1091020204

✦ Synopsis

FOUR,TEEN FIGURES

Formed protein elements of cells and tissues may be characterized by measuring the degree of their affinity for acid and basic dyes. For this purpose serial histological sections are stained at successive pH, other conditions being maintained constant (Singer and Morrison, '46 ; Dempsey and Singer, '46 ; Dempsey et al., '47). By this method human placentas of various ages have been investigated with reference to the staining characteristics of the trophoblastic syncytium, fibrin and fibrinoid.

Methods

Four human placentas, one of 5 weeks, two of 15 weeks and one of term, were investigated. Pieces of these placentas were fixed in Zenker 's acetic solution, sectioned at 5 p, mounted on slides and stained in most instances with either orange G (an acid dye) or methylene blue ( a basic dye). I n some cases, acid dyes other than orange G were used. Staining was carried out in large volumes of solution (1500 em3) so as to 'Reported a t the meeting of the American Association of Anatomists (Singer and Wislocki, '47).