The adsorption of lipids from the erythrocyte surface by silica and alumina

✍ Scribed by Brown, E. A.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1957
• Tongue: English
• Weight: 414 KB
• Volume: 50
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030500104

✦ Synopsis

During investigations of the effect of certain mercurated resins on the human erythrocyte surface, it was found that Wyflo-Super-Cel,' used as a supporting material in the column, rapidly haemolyzed the cells. This paper reports the results of experiments to dcterrriinc the nature of this haernolytic effect, and discusses its possible applications to the problem of the surface structure of the erythrocyte.

Outdated bank blood was the chief source of erythrocytes in these experiments, and on one occasion a bottle of blood only three days old, which had been rejected as unsafe to use for transfusion, was used. A suitable sample of the blood was removed, with sterile precautions, and centrifugcd a t 3,000 r.p.ni. to separate the plasnia. Thc cell deposit x i s resusperided in a n equal volume of :I RI/G solution of X.R. NaCl in glass-distilled water (' ' saliiie ") , centrifuged, this operation being repeated three times. Tlicn the cell deposit was transfcrrcd to a 500 ml centrifuge pot, and suspended in 500 ml of saline, centrifuged, and the supernatant discarded. The cell deposit was again resuspended in 500ml of KaC1 solution, centrifuged, and the supernatant rcmovcd and kept.

The washed cells mere now resuspended in 500ml saline.

Into a thoroughly cleaned glass tube 158cm long and 3 c m bore, closed off a t one end except for a n outlet tube, was placed 250ml of a 20% solution of sucrose. On top of this was floated the 500ml of cell suspension, and on this about Obtained from Johns-Manville Corp., U.S.A.