The adipokine SAA3 is induced by interleukin-1β in mouse adipocytes

✍ Scribed by Grit Sommer; Sebastian Weise; Susan Kralisch; Philipp E. Scherer; Ulrike Lössner; Matthias Blüher; Michael Stumvoll; Mathias Fasshauer

• Book ID: 102303846
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 103 KB
• Volume: 104
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.21782

✦ Synopsis

Abstract

Serum amyloid A (SAA) 3 has been characterized as an inflammatory adipocyte‐secreted acute‐phase reactant. In the current study, regulation of SAA3 by the proinflammatory and insulin resistance‐inducing cytokine interleukin (IL)‐1β was determined in 3T3‐L1 and brown adipocytes. Interestingly, SAA3 mRNA and protein synthesis were dramatically increased by IL‐1β in a time‐dependent fashion with maximal induction after 24 h. Furthermore, IL‐1β significantly induced SAA3 mRNA expression dose‐dependently with maximal 36.4‐fold upregulation seen at 2 ng/ml effector. Moreover, IL‐1β‐induced SAA3 expression was mediated by nuclear factor‐κB and janus kinase 2. Taken together, our data show a potent upregulation of SAA3 by IL‐1β. J. Cell. Biochem. 104: 2241–2247, 2008. © 2008 Wiley‐Liss, Inc.