The activity of powdery-mildew haustoria after feeding the host cells with different sugars, as measured with a potentiometric cyanine dye
✍ Scribed by Kurt Mendgen; Petra Nass
- Book ID
- 104754527
- Publisher
- Springer-Verlag
- Year
- 1988
- Tongue
- English
- Weight
- 541 KB
- Volume
- 174
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
The biotrophic parasite Erysiphe graminis f. sp. hordei produces haustoria within the cells of its host Hordeum vulgare. To determine the physiological activity of these haustoria, the electric potential across the membranes in the mitochondria of the haustorium was studied. The membrane potential was estimated with the fluorescent potentiometric cyanine dye 3,3'-dibutyloxacarbocyanine iodide. The addition of depolarizing agents (carbonylcyanide m-chlorophenylhydrazone, 2,4-dinitrophenol or KCN) to infected cells resulted in an increase of fluorescence after the addition of low concentrations or a decrease of fluorescence after the addition of higher concentrations. When the infected host cell was fed with increasing concentrations of D-glucose (25, 50, 75 mM), corresponding decreases of fluorescence were measured immediately in the mitochondria of the fungal haustoria. Sucrose induced a similar reduction of fluorescence about 20 min late. D-Galactose and D-fructose induced a somewhat smaller reduction of fluorescence, L-glucose and D-glucitol had no effect. The results indicate that haustoria take up glucose from the host cells immediately. Sucrose, D-galactose and D-fructose seem to require time to be metabolized before their products reach the fungal haustorium or mitochondria.