The activities of peroxisomal oxidases in periportal and perivenous zones of the rat liver acinus
β Scribed by M. Hir; U. C. Dubach
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 275 KB
- Volume
- 69
- Category
- Article
- ISSN
- 1432-119X
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β¦ Synopsis
Using a new biological microassay the activities of three peroxisomal oxidases in single microdissected periportal and perivenous zones of the liver acinus were measured. Whereas urate oxidase is homogeneously distributed through the acinus, the activities of D-aminoacid oxidase and alpha-hydroxyacid oxidase are respectively 1.80- and 2.71-fold higher in the periportal hepatocytes than in the perivenous hepatocytes.
π SIMILAR VOLUMES
Qualitative histochemical G6Pase distribution patterns obtained with an improved method (Teutsch, 1978) served as the basis for a zonal microdissection of the liver acinus. G6Pase activity was determined quantitatively in tissue samples of zones 1 and 3 by a microfluorometric method (Burch et al., 1
Periportal (pp) and perivenous (pv) hepatocyte populations were separated using a two-directional closed perfusion technique with selective addition of collagenase either to direct or retrograde perfusions (VÀÀnÀnen, H. et al., Liver 1983; 3:131). The activity of GPT in hepatocytes from the pp-area