The action of human C3 in soluble or cross-linked form with resting and activated murine B lymphocytes
✍ Scribed by Anna Erdei; Fritz Melchers; Thomas Schulz; Manfred Dierich
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 568 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
The third component of complement C3 has been implied in the stimulation of B lymphocytes to proliferation and maturation for Ig secretion. We have reinvestigated the extent of this activation with either activated or resting murine splenic lymphocytes in serum-substituted cultures. Human C3 was used in either soluble or crosslinked form. Soluble as well as Sepharose-bound or glutaraldehyde-cross-linked C3, over a range of concentrations, was inactive with resting splenic lymphocytes of (C57BW6J x DBAR)F,, C3H/HeJ and C57BL/6J nuinu mice. However, lipopolysaccharide-activated spleen cells, enriched for B cell blasts, were stimulated by immobilized and cross-linked C3, while they did not respond to soluble C3. The extent of restimulation was comparable to that indiced by lipopolysaccharide and resulted in both increased proliferation and maturation to Ig-secreting cells. The stimulation of the blast cells appears to be C3 specific, since it can be inhibited by free c3.