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The Action of Adenosine Deaminase (E.C. 3.5.4.4.) on Adenosine and Deoxyadenosine Acetates: The Crucial Role of the 5′-Hydroxy Group for the Enzyme Activity

✍ Scribed by Pierangela Ciuffreda; Silvana Casati; Enzo Santaniello

Publisher: Elsevier Science
Year: 2000
Tongue: French
Weight: 101 KB
Volume: 56
Category: Article
ISSN: 0040-4020
DOI: 10.1016/s0040-4020(00)00222-2

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✦ Synopsis

AbstractÐFrom adenosine 1, 2 H -deoxyadenosine 3 and 3 H -deoxyadenosine 5 all the acetates were prepared by lipase-catalyzed reactions. Only the acetates with free 5 H -hydroxy group were deaminated by adenosine deaminase (ADA), con®rming the crucial role of 5 H -OH for the enzyme activity.

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