The accumulation of podophyllotoxin-β-d-glucoside by cell suspension cultures derived from the coniferCallitris drummondii

✍ Scribed by Wim Uden; Niesko Pras; Theo M. Maingré

• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 352 KB
• Volume: 9
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00232296

✦ Synopsis

Podophyllotoxin was produced by cell cultures derived from needles of Callitris drummondii. The needles of this conifer contained 1.56% podophyllotoxin on a dry weight basis, 32% being present in the β-glucosidic form. Trace amounts of desoxypodophyllotoxin and matairesinol were also detected. In dark-grown cell cultures, ca. 0.02 % podophyllotoxin was accumulated, 85-90 % in the β-D-glucosidic form. Moreover, traces of the lignans matairesinol, hinokinin and asarinin were detected. Illumination stimulated the endogenous production of podophyllotoxin-β-D-glucoside; contents of up to 0.11 % could be measured.