The absence ofβ2-microglobulin in Daudi cells: Active gene but inactive messenger RNA
✍ Scribed by Claude Prévall; Bernard Mach
- Publisher
- Springer-Verlag
- Year
- 1983
- Tongue
- English
- Weight
- 511 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0093-7711
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✦ Synopsis
The Daudi cell line is characterized by an absence of HLA antigen on its surface. This has been attributed to a lack of ]~2-microglobulin (]?2m) while the heavy chain of HLA is present intracellularly. Karyotype analysis of Daudi cells has shown a deletion involving one of the ]?/-microglobulin alleles. It was generally believed that the absence of ]?2-microglobulin in that cell line resulted from an absence of expression of the remaining gene. We report here the unexpected finding of a normal amount of ]~2-microglobulin messenger RNA in Daudi cells. This was demonstrated by "Northern blot" hybridization with cDNA plasmid clones as a probe. This mRNA, however, when purified by hybridization-selection with /~2-microglobulin plasmid DNA, is unable to function as messenger in protein synthesis and is therefore an inactive mRNA. The finding ofa translationally inactive fl/-microglobulin mRNA provides a new explanation for the absence of ]~2-microglobulin and therefore of HLA antigens in Daudi cells.