The 49 K subunit of NADH: ubiquinone reductase (complex I) fromNeurospora crassa mitochondria: primary structure of the gene and the protein

✍ Scribed by D. Preis; J. C. Pas; U. Nehls; D. -A. Röhlen; U. Sackmann; U. Jahnke; H. Weiss

• Book ID: 104721672
• Publisher: Springer-Verlag
• Year: 1990
• Tongue: English
• Weight: 601 KB
• Volume: 18
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00321116

✦ Synopsis

The primary structure of the 49 K subunit of the respiratory chain NADH:ubiquinone reductase (complex I) from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the mature protein. The sequence lengths correlate to a molecular mass of 54,002 daltons for the preprotein and 49,239 daltons for the mature protein. The presequence consists of 42 amino acids of typical composition for sequences which target nuclear-encoded proteins into mitochondria. The mature protein consists of 436 amino acids and shows 64% similarity to a 49 K subunit of bovine heart NADH:ubiquinone reductase and 33% to a predicted translation product of an open reading frame in the chloroplast DNAs of Marchantia polymorpha and Nicotiana tabacum. Evidence for an iron-sulfur cluster in the subunit is discussed.