Thaumatin-like protein in kiwifruit

✍ Scribed by Wurms, Kirstin; Greenwood, David; Sharrock, Keith; Long, Peter

• Book ID: 101225853
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 178 KB
• Volume: 79
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(199908)79:11<1448::aid-jsfa381>3.0.co;2-3

✦ Synopsis

A 21-kDa protein extracted from woody tissue underlying the pedicel of kiwifruit (Actinidia deliciosa var deliciosa [A Chev] Liang and Ferguson) was puri®ed to homogeneity by cation exchange, gel ®ltration, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) on a 15% gel and electro-transfer onto a polyvinylidene di¯uoride (PVDF) membrane, followed by excision of the band of interest. Amino terminal sequence analysis of this band revealed a high degree of homology with basic endochitinase (EC 3.2.1.14) in orange and thaumatin-like proteins in tobacco, barley, maize and tomato. This is the ®rst record of a thaumatin-like protein in a subtropical berryfruit. Potential implications of this ®nding and the need for future research are discussed.