Th1-type cytokines production is decreased in kidney transplant recipients with active cytomegalovirus infection
✍ Scribed by Essa, Sahar; Raghupathy, Raj; Pacsa, Alexander S.; El-Shazly, Amany; Said, Tareq; Azizieh, Fawaz
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 174 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Cytomegalovirus (CMV) infection is a major complication after kidney transplantation. Despite antiviral therapy the infection contributes significantly to high morbidity. The present study was aimed at determining: (a) the stimulation index (S.I.) of phytohemagglutinin (PHA)stimulated peripheral blood mononuclear cells (PBMC) and (b) the levels of Th1-and Th2-related cytokines in kidney transplant recipients with and without active CMV infection. Thirtyfive patients with, and 44 without active CMV infections, as diagnosed by a CMV antigenemia assay, were inducted into this study. After PHA stimulation of PBMC from patients, stimulation index (S.I.) was determined by radioactive thymidine uptake while the production of Th1-type cytokines (interleukin-2 [IL-2], interferon-␥ [IFN-␥], and tumor necrosis factor-␣ [TNF-␣]) and Th2-type cytokines (IL-4, IL-10) were measured by enzyme-linked immunosorbent assay. PBMC of patients with active CMV infection showed significantly lower S.I. values than patients without an ongoing CMV infection (P < .0001). Levels of Th2-type cytokines in CMV-infected and uninfected kidney recipients were similar; however, the levels of the Th1-type cytokines were significantly lower in CMV-infected patients. Low levels of Th1-type cytokines seem to correlate well with active CMV infection in kidney recipients.