Th1 and Th2 CD4+ T cell clones specific for Plasmodium chabaudi but not for an unrelated antigen protect against blood stage P. chabaudi infection
✍ Scribed by Andrew W. Taylor-Robinson; R. Stephen Phillips
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 800 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
The host protective immune response to blood stage malaria infection was studied using Plasmodium chabaudi chabaudi (Echabaudi) in NIH mice. It has been shown previously that CD4+ cells are critically required for protection against erythrocytic infection. Mice lacking a functional CD4+ cell compartment suffer unremitting patent primary parasitemias for at least 60 days after infection. Here, we report that the adoptive transfer of eight f? chabaudi-specific CD4+ T cell clones of either the Thl or Th2 type to mice rendered CD4-depleted by adult thymectomy and anti-CD4 monoclonal antibody therapy fully restored the ability of recipients to control challenge infection. Control Thl and Th2 clones specific for an unrelated antigen, ovalbumin, were unable to confer a comparable level of protection in CDCdepleted mice, even though they received regular doses of the antigen. These data demonstrate that protective immunity to asexual €? chabaudi parasites can be mediated through immune CD4+ T cell clones of either theTh1 or the Th2 subset.
Recent studies suggest an important role for CD4+ T cells in the protective immune response to l ? chabaudi. Brake et al.
[6] showed that a CD4+ Tcell clone, of apparent Thl-type, transferred protection against the related parasite l? chabaudi adami. For P chabaudi itself, limiting dilution analysis revealed a functional heterogeneity of the CD4+ cell precursor frequency following infection and provided indirect evidence that effector mechanisms controlling the acute primary parasitemia may be predominantly Abindependent but are superseded by Ab-mediated immunity . To explore this possibility by a different approach, we established cloned CD4+ Tcell lines which react to l? cha- [I 118721