TGF-β-induced cell-cycle arrest through the p21WAF1/CIP1-G1 cyclin/Cdks-p130 pathway in gastric-carcinoma cells
✍ Scribed by Young D. Yoo; Ju-Youn Choi; Su-Jae Lee; Jun S. Kim; Byung-Re Min; Young I. Lee; Yoon-Koo Kang
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- French
- Weight
- 211 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Transforming growth factor-1 (TGF-) inhibits cell-cycle progression of many types of cells by arresting them in G
1 /S phase through inhibition of the active cyclin-Cdk complexes that lead to inhibition of Rb phosphorylation. In gastriccancer cells, SNU16, TGF- treatment induced enhanced expression of p21 WAF1/CIP1 (p21), which inhibited the kinase activity of cyclin-D-and cyclin-E-associated Cdks and blocked p130 phosphorylation. TGF- also enhanced the stability of p130, suggesting that hypophosphorylation of p130 and increased stability of p130 contribute to p130-mediated G 1 arrest in gastric-cancer cells. Our results demonstrate that p21 and p130 are major downstream targets of TGF- in gastric-cancer cells and that a p21-G 1 cyclin/Cdks-p130/E2F pathway mediates growth inhibition by TGF- in these cells.