Tetraphenylphosphonium chloride induced mr-visible lipid accumulation in a malignant human breast cell line

✍ Scribed by Edward J. Delikatny; Sandrine K. Roman; Rebecca Hancock; Thomas M. Jeitner; Catherine M. Lander; Darryl C. Rideout; Carolyn E. Mountford

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 825 KB
• Volume: 67
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19960703)67:1<72::aid-ijc13>3.0.co;2-e

✦ Synopsis

The effect of the cationic lipophilic phosphonium salt tetraphenylphosphonium chloride (TPP) on a human malignant breast cell line, DU4475, was monitored with proton nuclear magnetic resonance (IH MRS). TPP caused a dose-and timedependent increase in resonances arising from MR-visible lipid as measured by the CH2/CH3 ratio in the I-dimensional 'H MR spectrum. Two-dimensional MRS identified increases in the glycerophosphocholine/ Iysine cross-peak ratio and corresponding decreases in the phosphocholine/lysine ratio in a dosedependent fashion in TPP-treated cells. Lipid metabolic changes are discussed in the light of other MR experiments, and the data indicate that accumulation of MR-visible lipids may arise from the rearrangement of phospholipids accompanying mitochondrial destruction or from the catabolism of phospholipids associated with early events in the cytotoxic process.