Tetranitromethane modification of photosystem 2

✍ Scribed by C. Walczak; S. Kumar; J. T. Warden

• Book ID: 104623118
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 516 KB
• Volume: 12
• Category: Article
• ISSN: 0166-8595
• DOI: 10.1007/bf00047944

✦ Synopsis

Inhibition of photosystem 2 by the peptide-modification reagent, tetranitromethane, has been investigated with spinach digitonin particles. In the presence of tetranitromethane, (1) the initial fluoresence yield is suppressed with a concomitant elimination of the variable component of fluorescence; (2) the optical absorption transient at 820 nm, attributed to P680(+), is greatly attenuated; (3) diphenylcarbazide-supported photoreduction of dichlorophenol indophenol is abolished; and (4) electron spin resonance Signal 2f and Signal 2s are eliminated. These results are consistent with multiple sites of modification in photosystem 2 by tetranitromethane, and suggest further that this reagent can inhibit charge stabilization in the reaction center.