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Tetracycline-regulatable expression vectors tightly regulate in vitro gene expression of secreted proteins

✍ Scribed by Franck G. Sturtz; Linda Cioffi; Susan Wittmer; Matthew J. Sonk; Alan Shafer; Yunsheng Li; Nicholas J. Leeper; Julie Smith-Gbur; Janine Shulok; Doros Platika

Book ID
117523327
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
201 KB
Volume
221
Category
Article
ISSN
0378-1119

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A set of Saccharomyces cerevisiae expression vectors has been developed in which transcription is driven by a hybrid tetO-CYC1 promoter through the action of a tetR-VP16 (tTA) activator. Expression from the promoter is regulated by tetracycline or derivatives. Various modalities of promoter and acti