Testing the LightCycler Candida Kit MGRADE in artificially-contaminated whole blood and serum: Consequences for the diagnosis of invasive candidiasis
✍ Scribed by C. Estran; F. Durand; M.P. Brenier-Pinchart; A. Bas; N. Ravanel; B. Lebeau; J.P. Brion; H. Pelloux; R. Grillot
- Publisher
- Masson Editeur
- Year
- 2008
- Tongue
- French
- Weight
- 174 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1156-5233
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✦ Synopsis
Fungal infections are a cause of morbidity and mortality in immunocompromised patients. Among fungal infections, Candida albicans is the most frequent. A standardized method to detect C. albicans in whole blood or serum with LightCycler Candida Kit M GRADE was tested for the first time. Whole blood and serum were artificially infected with C. albicans cells or DNA, respectively. Sensitivity was 5 Â 10 2 CFU/ml in whole blood and five copies of genome per milliliter in serum. None commercially available real-time PCR kit has been reported yet to detect C. albicans and it is the first time that such a kit is described to detect C. albicans in biological samples. However, interest is limited because the kit LightCycler Candida Kit M GRADE only detects C. albicans.