Testing homogeneity of intra-run variance parameters in immunoassay

✍ Scribed by Qi Zeng; Marie Davidian

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 235 KB
• Volume: 16
• Category: Article
• ISSN: 0277-6715
• DOI: 10.1002/(sici)1097-0258(19970815)16:15<1765::aid-sim603>3.0.co;2-p

✦ Synopsis

A common assumption in the analysis of immunoassay data is a similar pattern of within-run variation across runs of the assay. One makes this assumption without formal investigation of its validity, despite the widely acknowledged fact that accurate understanding of intra-run variation is critical to reliable calibration inference. We propose a simple procedure for a formal test of the assumption of the homogeneity of parameters that characterize intra-run variation based on representation of standard curve data from multiple assay runs by a non-linear mixed effects model. We examine the performance of the procedure and investigate the robustness of calibration inference to incorrect assumptions about the pattern of intra-run variation.