Testicular germ cell tumors of adults show deletions of chromosomal bands 11 p13 and 11p15.5, but no abnormalities within the zinc-finger regions and exons 2 and 6 of the wilms' Tumor 1 Gene
✍ Scribed by Dr. Leendert H. J. Looijenga; Ad J. M. Gillis; J. Wolter Oosterhuis; Mary Abraham; Grady F. Sunders
- Book ID
- 102845509
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 669 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
We have studied the involvement of chromosomal bands I I p I 3 and I I p 15.5 in I 5 testicular seminomas (SE) and I8 testicular nonseminomatous germ cell tumors (NS). NO allelic imbalances were found in 40% of the SE and 44% of the NS. LOSS of heterozygosity (LOH) at I I p 15.5 was seen in 2 I % of the SE and 47% of the NS; the corresponding frequencies for I I p I 3 were 47% and 44%. Both regions were deleted in 13% of the SE and 44% of the NS, indicating that all NS with a complete LOH of I I p I 3 also lost the I I p 15.5 region. In one (out of two) SE and in five (out of eight) NS, this was due to at least two separate deletions. Loss of the whole p-arm was likely in one SE and two NS. No gross genomic changes of the Wilms' tumor I (WTI) tumor suppressor gene were found using a cDNA probe (WT33). Nor were aberrations found in the zinc-finger regions and exons 2 and 6 of this gene, using polymerase chain reaction amplification, single stranded DNA polymorphism analysis, and sequencing. We suggest that loss of genetic information from the short arm of chromosome I I, without affecting the W1 gene in the regions studied, is relatively frequent but not crucial in the pathogenesis of testicular germ cell tumors of adults. Genes Chrom Cancer 9:153-160 (1994).