Test paper for detection of lipoxygenase

✍ Scribed by Benito O. de Lumen; Stanley J. Kazeniac

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 562 KB
• Volume: 99
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90051-4

✦ Synopsis

A quick, simple, and specific test for lipoxygenase was developed. Filter paper impregnated with linoleic acid and N,N'-dimethyl-p-phenylenediamine gave a blue color as a positive test. The specificity of the test was established by: use of inhibitors of lipoxygenase and heme-catalyzed peroxidation, use of nonsubstrates, use of inactivated enzymes, proportionality of color intensity to amount of enzyme applied, and use of nonoptimum pH. Heattreated tomato samples showed decreasing color intensities with increasing temperatures. The technique should prove useful as a quality control tool in the quick detection of lipoxygenase during processing and especially in in situ localization of the enzyme in fruits and vegetables.