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Tertiary Windowing to Detect Positive Diversifying Selection

✍ Scribed by Ann-Charlotte Berglund; Björn Wallner; Arne Elofsson; David A. Liberles


Publisher
Springer
Year
2005
Tongue
English
Weight
137 KB
Volume
60
Category
Article
ISSN
0022-2844

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We have substituted deoxyadenosine or adenosine for hypoxanthine in t h e standard HAT selection system in an attempt to select for ADA-normal (ADA+) cells. ADA-human lymphoid line cells could not utilize deoxyadenosine as an alternative to hypoxanthine as a purine source (DAT) and failed to grow bu