Terminal differentiation of spontaneous rheumatoid factor–secreting B cells from rheumatoid arthritis patients depends on endogenous interleukin-1 0
✍ Scribed by Lisardo Perez; Javier Orte; José A. Brieva
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 732 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
The presence of serum rheumatoid factor (RF) and spontaneous RF-secreting B cells is a common feature in most patients with rheumatoid arthritis (RA). This study analyzed the cytokine(s) that controls the final maturation of B cells capable of spontaneous IgM-RF secretion in vitro.
Methods. RA patients' peripheral blood mononuclear cells (PBMC), as well as adherent and nonadherent cell fractions, were cultured, and spontaneous IgM-RF and interleukin-10 (IL-10) secretion were determined by enzyme-linked immunosorbent assay.
Results. The RF+ RA PBMC, but not PBMC from RF-RA patients or healthy controls, actively produced IgM-RF in a linear manner for 14 days. This activity depended on the presence of fetal calf serum and did not require cellular DNA synthesis.