Teratogenicity Testing

✍ Scribed by Luís Félix

• Publisher: Springer New York;Humana Press
• Year: 2018
• Tongue: English
• Leaves: 603
• Series: Methods in Molecular Biology 1797
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Teratogenicity Testing: Methods and Protocols describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.

✦ Table of Contents

Front Matter ....Pages i-xv
Front Matter ....Pages 1-1
An Overview of Teratology (Ana M. Calado, Maria dos Anjos Pires)....Pages 3-32
Teratology Study Guidelines: An Overview (Sofia Alves-Pimenta, Luís Félix, Bruno Colaço, Paula A. Oliveira, Carlos Venâncio)....Pages 33-59
Biological Concerns on the Selection of Animal Models for Teratogenic Testing (Sofia Alves-Pimenta, Bruno Colaço, Paula A. Oliveira, Carlos Venâncio)....Pages 61-93
Front Matter ....Pages 95-95
The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells (Lauren M. Walker, Darcie L. V. McClelland-Descalzo, Nicole I. zur Nieden)....Pages 97-124
Human Pluripotent Stem Cells to Assess Developmental Toxicity in the Osteogenic Lineage (Joseph V. Madrid, Steven R. Sera, Nicole R. L. Sparks, Nicole I. zur Nieden)....Pages 125-145
Chick Embryonic Cardiomyocyte Micromass System for Assessing Developmental Cardiotoxicity of Drugs (Wasay Mohiuddin Shaikh Qureshi, Margaret K. Pratten)....Pages 147-163
Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell (Valéria Maria Lara, Kelly C. Santos Roballo, André Tadeu Gotardo, Carlos Eduardo Ambrósio)....Pages 165-175
Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs (Bo Liang, Chi Chiu Wang)....Pages 177-189
Western Blot Methodologies for Analysis of In Vitro Protein Expression Induced by Teratogenic Agents (Carlos Martins-Gomes, Amélia M. Silva)....Pages 191-203
Manipulation of MicroRNAs in Cultured Mouse Embryos: Applications for Developmental Toxicology (Fuqiang Yuan, Shao-yu Chen)....Pages 205-214
Front Matter ....Pages 215-215
Insights into the Phenotypic and Behavioral Effects of Teratogenic Drugs in Caenorhabditis elegans (Caralina Marín de Evsikova)....Pages 217-232
Effect of Teratogens on Development of Drosophila melanogaster (Dong Li, Xiaolin Bi)....Pages 233-241
Cellular Responses in Drosophila melanogaster Following Teratogen Exposure (Matheus C. Bianchini, José L. R. Portela, Robson L. Puntel, Daiana S. Ávila)....Pages 243-276
Behavioral Teratogenesis in Drosophila melanogaster (Monalisa Mishra, Bedanta Kumar Barik)....Pages 277-298
Evaluation of Teratogenicity of Pharmaceuticals Using FETAX (Hariz Islas-Flores, Itzayana Pérez-Alvaréz, Leobardo M. Gómez-Oliván)....Pages 299-307
Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays (Mark E. Pownall, Margaret S. Saha)....Pages 309-323
Visualization of Gene Expression Patterns by In Situ Hybridization on Early Stages of Development of Xenopus laevis (Heithem M. El-Hodiri, Lisa E. Kelly)....Pages 325-335
Analysis of Lethality and Malformations During Zebrafish (Danio rerio) Development (Azhwar Raghunath, Ekambaram Perumal)....Pages 337-363
General Whole-Mount Immunohistochemistry of Zebrafish (Danio rerio) Embryos and Larvae Protocol (Dércia Santos, Sandra M. Monteiro, Ana Luzio)....Pages 365-371
Geometric Morphometrics as a Tool to Evaluate Teratogenic Effects in Zebrafish (Danio rerio) (Kathryn E. Perez, Tisha C. King-Heiden)....Pages 373-391
Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer (Simon T. Bond, Kevin A. McEwen, Prusothman Yoganantharajah, Yann Gibert)....Pages 393-401
Behavioral Profiling of Zebrafish (Danio rerio) Larvae Following Teratogen Exposure (Ana M. Valentim)....Pages 403-419
Omics in Zebrafish Teratogenesis (Benjamin Piña, Laia Navarro, Carlos Barata, Demetrio Raldúa, Rubén Martínez, Marta Casado)....Pages 421-441
Proteomic Analysis of Zebrafish (Danio rerio) After Chemical Exposure (Yew Mun Lee, Caixia Li, Siew Hong Lam, Zhiyuan Gong, Qingsong Lin)....Pages 443-459
Immunohistochemical Assessment as a Tool for Investigating Developmental Toxicity in Zebrafish (Danio rerio) (Carla Santos, Maria de Lurdes Pinto)....Pages 461-476
Oxidative Stress Assessment in Zebrafish Larvae (Inês Domingues, Carlos Gravato)....Pages 477-486
Hemodynamic Studies for Analyzing the Teratogenic Effects of Drugs in the Zebrafish Embryo (Huseyin C. Yalcin)....Pages 487-495
Western Blot Analysis and Immunostaining for Prediction of Embryotoxicity in Mus musculus (Yulia Irnidayanti, Win Darmanto, Dias R. Sutiono)....Pages 497-505
Histological and Histochemical Profile for Teratological Assessment in Mus musculus (Maria de Lurdes Pinto, Paula Rodrigues)....Pages 507-530
In Vivo Analysis of Apoptosis in Embryonic Hippocampus (Ahmad Salimi, Jalal Pourahmad)....Pages 531-536
Measurement of Mitochondrial Toxicity Parameters in Embryonic Hippocampus (Ahmad Salimi, Jalal Pourahmad)....Pages 537-544
Animal Tests for Evaluation of Cognitive Impairment in Neonatal Mouse (Ahmad Salimi, Jalal Pourahmad)....Pages 545-554
Methodology of Genotoxic and Teratogenic Studies in Rats (Eyyup Rencuzogullari, Muhsin Aydin)....Pages 555-575
Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos (Yoshinori Kawamura, Kiyoshi Matsumoto)....Pages 577-594
Back Matter ....Pages 595-614

✦ Subjects

Biomedicine; Pharmacology/Toxicology