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Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse

✍ Scribed by Michael Schuler; Faisal Ali; Elisabeth Metzger; Pierre Chambon; Daniel Metzger

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
604 KB
Volume
41
Category
Article
ISSN
1526-954X

No coin nor oath required. For personal study only.

✦ Synopsis

To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA-Cre-ER(T2) mouse line in which the expression of the tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the human skeletal muscle alpha-actin gene, contained in a P1-derived artificial chromosome. In this transgenic line Cre-ER(T2) is selectively expressed in skeletal muscles, and Cre-ER(T2)-mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle-specific and strictly tamoxifen-dependent. HSA-Cre-ER(T2) mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders.

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