Temporal specificity of hemoglobin synthesis in the fat body ofChironomus thummi during development

✍ Scribed by Schin, Kissu ;Laufer, Hans ;Clark, Ralph M.

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 734 KB
• Volume: 210
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402100210

✦ Synopsis

Abstract

Hemoglobin (Hb) synthesis in the subepidermal fat body cell during the development of Chironomus thummi was investigated by using positive diamino‐benzidine (DAB) reaction as an indicator for the presence of Hb. The localization of DAB reaction products in the ER and Golgi cisternae is stage‐specific. Active synthesis of Hb, as indicated by heavy deposits of reactive products, is interrupted during third‐fourth larval ecdysis, resumes about the fourth day of the fourth instar, continues throughout the fourth instar and stops again shortly before the transition from the pharate pupa to the pharate adult at which time the fat body shows numerous autophagic vacuoles. These results support the concept of the existence of specific switches in the production, release and degradation of Hb present in Chironomus.