Template-directed reversible photocircularization of DNA via 5-vinyldeoxycytidine

✍ Scribed by Kenzo Fujimoto; Shigeo Matsuda; Naoki Ogawa; Masayuki Hayashi; Isao Saito

• Book ID: 104210647
• Publisher: Elsevier Science
• Year: 2000
• Tongue: French
• Weight: 153 KB
• Volume: 41
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(00)01025-x

✦ Synopsis

An ecient and reversible template-directed photoligation of oligodeoxynucleotides (ODNs) using 5-vinyldeoxycytidine ( V C) was described. When ODN containing V C at the 5 H end was photoirradiated with ODN containing thymine at the 3 H end in the presence of a template ODN, an ecient photoligation was observed. Reversible photopadlocking of a circular DNA by the photocircularization using V C was demonstrated.