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Temperature-sensitive expression of auxin-autotrophy by crown-gall teratoma cells of tobacco

โœ Scribed by Frederick Meins

Book ID
104749342
Publisher
Springer-Verlag
Year
1975
Tongue
English
Weight
531 KB
Volume
122
Category
Article
ISSN
0032-0935

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โœฆ Synopsis

Crown-gall teratoma tissues of tobacco (Nicotiana tabacum L.) grow in culture at 25ยฐ in the absence of added auxin or cell-division factors. The capacity of these tissues to grow without added auxin is temperature sensitive. At 35ยฐ auxin provided as ฮฑ-naphthalene acetic acid or 2,4-dichlorophenoxyacetic acid is required for continued exponential growth. Moreover at 35ยฐ the auxin dose response of teratoma tissues shifts from the pattern characteristic of autonomous tumor tissues at 25ยฐ to the pattern characteristic of auxin-requiring normal tissues. Incubation of the tissues at 35ยฐ also appears to induce lasting changes in teratoma phenotype. Using relative growth of tissues with and without added auxin as a criterion, pretreatment with auxin induces a stable increase in auxin-autotrophy, while pretreatment without auxin induces a stable decrease in auxin-autotrophy. These results are consistent with a positive feedback mechanism for stable changes in auxin-autotrophy.

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