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Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering

✍ Scribed by Masayuki Yamato; Yoshikatsu Akiyama; Jun Kobayashi; Joseph Yang; Akihiko Kikuchi; Teruo Okano

Book ID: 104090323
Publisher: Elsevier Science
Year: 2007
Tongue: English
Weight: 866 KB
Volume: 32
Category: Article
ISSN: 0079-6700
DOI: 10.1016/j.progpolymsci.2007.06.002

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✦ Synopsis

We have developed a novel strategy for regenerative medicine to recover tissue functions by using temperatureresponsive cell culture surfaces. To overcome the limits of conventional methods such as the use of single-cell suspension injection and the use of biodegradable polymer scaffolds, we have applied transplantable cell sheets fabricated with temperature-responsive culture surfaces for cell delivery. Here, we summarize the development of the next generation of temperature-responsive cell culture surfaces using modifications of the polymer coating to allow for rapid cell sheet recovery; improved culture conditions, such that animal-derived products can be eliminated; and altered phase transition temperatures to produce patterned cell sheets. These designs allow for the reconstruction of more complex tissues to potentially treat a wide range of diseases.

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