Temperature response of the cell cycle ofHaplopappus gracilisin suspension culture and its significance to the G1transition probability model

✍ Scribed by Alan R. Gould

• Book ID: 104754304
• Publisher: Springer-Verlag
• Year: 1977
• Tongue: English
• Weight: 717 KB
• Volume: 137
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00394431

✦ Synopsis

The effects of temperature on the cell cycle of Haplopappus gracilis suspension cultures were analysed by the fraction of labelled mitoses method. Sphase in these cultures shows a different temperature optimum as compared to optima derived for G2 and mitosis. G1 phase has a much lower Q10 than the other cell cycle phases and shows no temperature optimum between 22 and 34° C. These results are discussed in relation to a transition probability model of the cell cycle proposed by Smith and Martin (Proc. Natl. Acad. Sci. USA 70, 1263-1267, 1973), in which each cell has a time independent probability of initiating the transition into another round of DNA replication and division. The implications of such a model for cell cycle analysis are discussed and a tentative model for a probabilistic transition trigger is advanced.