Temperature-pressure studies on the cardiac rate in tissue culture explants from the heart of the tadpole (Rana pipiens)
✍ Scribed by Landau, Joseph ;Marsland, Douglas
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1952
- Tongue
- English
- Weight
- 734 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0095-9898
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✦ Synopsis
Especially since 1934, high pressure has provided a very useful method for analyzing physiological processes in various cells and tissues (cf. Marslartd, '42) ; and studies in which both temperature and pressure have served as experimental variables have been even more revealing (cf. Brown, '34; Brown, Johnson and Marsland, '42 ; Marsland, '50). However, these techniques have nol, previously been applied to tissue culture preparations.
Explants of various tissues from young frog tadpoles were tested as to viability and growth under tissue culture conditions and cardiac tissue was finally chosen for the experiments. This tissue proved to be exceptionally hardy under the experimental conditions (see later). Also, the steady rhythmic beating of the heart fragments, which continued more or less indefinitely in the cultures, provided an opportunity for studying the cardiac rate at temperatures between 5" to 40"C., and at pressures ranging up to 16,000 lbs./in.2 (Landau and Marsland, '50). .And finally, previous studies dealing with pressure effects on rate processes -particularly the work of Edwards and Cattell ('28) on the cardiac rate of the intact heart (frog and turtle), and that of Pease and