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Temperature gradient focusing in miniaturized free-flow electrophoresis devices

✍ Scribed by Marco Becker; Abraham Mansouri; Cornelia Beilein; Dirk Janasek

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 570 KB
Volume: 30
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200900359

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✦ Synopsis

Temperature gradient focusing in miniaturized free-flow electrophoresis devices

Temperature gradient focusing is a method to separate and focus any charged analytes even without accessible isoelectric point, and has been already widely used in CE. In this paper, we demonstrate the application of temperature gradient focusing to free-flow electrophoresis. Besides focusing and separation experiments of proteins, the stability of the temperature gradient under flow conditions and the temperature dependence of fluorescence dyes have also been investigated.

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