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Temperature effects on separation on zirconia columns: Applications to one- and two-dimensional LC separations of phenolic antioxidants

✍ Scribed by Francesco Cacciola; Pavel Jandera; Luigi Mondello

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
910 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

The effects of temperature and mobile phase on LC chromatographic separation of phenolic antioxidants on zirconia‐based columns were investigated. Unlike silica‐based materials, zirconia columns show excellent thermal stability over a wide range of temperatures and enable high‐temperature separations. Enthalpic and entropic contributions to the retention of phenolic compounds on ZR‐Carbon and ZR‐Carbon C18 columns were determined from retention versus temperature plots in order to elucidate the retention mechanism of sample compounds over the temperature range up to 140°C. High‐temperature liquid chromatography on ZR‐Carbon columns was used for comprehensive LC×LC two‐dimensional separation systems based on the different selectivity of a Zorbax SB micro‐column used in the first dimension and a ZR‐Carbon column used in the second dimension. Two‐dimensional LC×LC systems employing a setup with two alternately operated parallel ZR‐Carbon columns in the second dimension were used for the analysis of phenolic antioxidants in beer and wine samples.

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