Temperature effects for chiral separations using various bulk fluids in near-critical mobile phases

✍ Scribed by John A. Blackwell; Rodger W. Stringham

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 91 KB
• Volume: 9
• Category: Article
• ISSN: 0899-0042
• DOI: 10.1002/(sici)1520-636x(1997)9:7<693::aid-chir10>3.0.co;2-i

✦ Synopsis

As supercritical fluid chromatography becomes more accepted as a facile means for the separation of chiral compounds, the need for mobile phases that can readily solubilize these polar compounds grows. Prior studies suggest that HFC-134a may prove suitable due to its very high eluotropic strength compared to carbon dioxidebased mobile phases. A comparison is made between ethanol-modified carbon dioxide, HFC-134a, and decafluoropentane as to their relative eluotropic strength, selectivity, and efficiency for three chiral compounds using a Whelk O-1 chiral bonded phase. The bulk component of the mobile phase was found to have relatively little effect on chiral selectivity over the range of 5°to 95°C.