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Temperature-dependent properties of cells transformed by a thermosensitive mutant (TS-121) of polyoma virus. II. Characterization of 121-6 cells

✍ Scribed by Yasuhiro S. Okada; Akira Hakura

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
832 KB
Volume
16
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The properties of 121‐6 cells were characterized. This cell line is transformed by ts‐121, a temperature‐sensitive mutant of polyoma virus. Both the morphology and saturation density of the 121‐6 cells were affected by temperature; i.e. at 39° C, the cells grew to monolayer sheets and remained contact inhibited for a long time (at least 25 days), while at 35° C, they grew beyond the monolayer density, like cells transformed by wild‐type polyoma virus. These phenotypic changes were reversible, but increased agglutinability by Concanavalin A, which was another phenotype of these transformed cells, was not temperature‐sensitive, and even at high temperature, 121‐6 cells, like transformed cells, showed high agglutinability.

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