Temperature Dependent Migration of the Nitro Group of Potassium 4-Amino-3,5-dinitrobenzenesulfonate vs Desulfonation.

Minimizing loss of sequence information

Minimizing loss of sequence information in SAGE ditags by modulating the temperature dependent 3′ → 5′ exonuclease activity of DNA polymerases on 3′-terminal isoheptyl amino groups

✍ Austin P. So; Robin F.B. Turner; Charles A. Haynes 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 343 KB 👁 2 views

## Abstract Numerous steps are required to prepare a sequencing library for serial analysis of gene expression (or SAGE) from an original mRNA sample. The presence of inefficiencies, however, can lead to a cumulative loss of sample during processing which can yield a library of short sequence tags