Temperature-dependent actinomycin D effect on RNA synthesis during synchronous development inNeurospora crassa

✍ Scribed by R. E. Totten; H. Branch Howe

• Publisher: Springer
• Year: 1971
• Tongue: English
• Weight: 657 KB
• Volume: 5
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00485670

✦ Synopsis

This concentration also inhibited the development of conidiophores and conidia at 35 C, but not at 30 or 25 C. Mycelia which were induced to synchronous development formed conidiophores in 2.5 hr and conidia in 4.5 hr at 35 C in the absence of drug additives. Addition of actinomycin D to synchronously developing mycelia at zero time and at 0.5-hr intervals thereafter at 35 C indicated that RNA synthesis required for conidiophores occurred before 0.5 hr and for eonidia before 2 hr. Addition of cycloheximide at the same times to another synchronous mycelial series at 35 C indicated that protein synthesis required for conidiophores occurred before 2 hr and for conidia before 3.5-4 hr. Actinomycin D (5 pg/ml of medium, vial cultures) Cycloheximide (5/lg, Petri dish cultures) 0 No No 0.5 Yes No