Temperature dependence of ultrasound-induced cell killing: The role of membrane fluidity
✍ Scribed by E. Ben-Hur; M. Green
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 280 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0197-8462
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✦ Synopsis
Abstract
Chinese hamster cells in suspension were exposed to 20 kHz ultrasound (US) at 54 W/cm^2^ and various temperatures between 2 and 44 °C. Activation energies were 2.6 and 24 kcal/mole below and above 35 °C, respectively. Procaine, a local anaesthetic drug known to increase membrane fluidity, enhanced cellular inactivation by US above 41 °C, increasing the activation energy to 62 kcal/mole. The inactivation of the bacterium Salmonella typhimurium by US was also dependent on the exposure temperature, with an activation energy of 2.9 kcal/mole between 2 and 44 °C. These data are most simply explained by the hypothesis that membranes are a major target for cellular inactivation by US and that the fluidity of the membranes is important in this respect.