✍ Scribed by Guy Beauregard; Michel Potier
No coin nor oath required. For personal study only.
The radiation inactivation method allows determination of the relative molecular mass (M,) of proteins by exposure to high doses of ionizing radiation. The analysis by target theory of biological activity decay curves yields the size of the protein. A correction factor for M, has been routinely used in the literature when irradiation is conducted at low temperature. Since the radiation inactivation of proteins is affected by temperature, we propose a general equation which relates IV, of a protein to L& the dose in megarads at a given temperature t (in "C) where 37% of its initial biological activity remains log M, = 5.89 -log D3,,, -0.0028t. It is concluded that temperature affects the amount of absorbed radiation energy required to inactivate 1 mol of protein.
📜 SIMILAR VOLUMES
## Abstract The change of the apparent partial specific volumes of egg albumin, bovine serum albumin, bovine methemoglobin, β‐lactoglobulin, and lysozyme with temperature through the thermal transitions of the proteins have been measured with dilatometers. Four regions in the plot of the apparent p
## Abstract The temperature dependence of the apparent expansibility of lysozyme and ovalbumin in Solution has been measured as a function of pH. This temperature dependence is explained in terms of suppressed fluctuations in bound water due to the protein. It is shown that the thermal expansion co
A peptide affinity inactivator, Ac-Leu-Arg-Arg-Ala-(BrAc)Om-Leu-Gly, was used as a tool t o probe for active site residues in the catalytic subunit of bovine CAMP-dependent protein kinase. The peptide inactivated the catalytic subunit in an active site-directed and monophasic manner with a first-ord
## Abstract Temperature‐sensitive imprinted and non‐imprinted hydrogels composed of __N__‐isopropylacrylamide (NIPA) and 2‐acrylamido‐2‐methyl‐propanosulfonic acid (AMPS) have been prepared by free‐radical crosslinking copolymerization in aqueous solution at three different temperatures: 10 °C (bel
High-density polyethylene (HDPE) and low-density polyethylene (LDPE) were irradiated in vacuo at 30 -220 and 30 -360°C, respectively, with ␥-rays at doses of 10 -400 kGy. Temperature dependence of cross-linking and gas evolution was investigated. It was found that cross-linking was the predominant p