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Temperature Dependence of the Protein-Chromophore Hydrogen Bond Dynamics in the Far-Red Fluorescent Protein Mplum

✍ Scribed by Regmi, Chola K.; Chapagain, Prem P.; Gerstman, Bernard S.

Book ID
122797301
Publisher
Biophysical Society
Year
2014
Tongue
English
Weight
39 KB
Volume
106
Category
Article
ISSN
0006-3495

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## Abstract mPlum is a far‐red fluorescent protein with emission maximum at ∼650 nm and was derived by directed evolution from DsRed. Two residues near the chromophore, Glu16 and Ile65, were previously revealed to be indispensable for the far‐red emission. Ultrafast time‐resolved fluorescence emiss