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Temperature dependence of the hydrogen ion equilibria in poly(riboadenylic acid)

✍ Scribed by D. N. Holcomb; Serge N. Timasheff

Publisher
Wiley (John Wiley & Sons)
Year
1968
Tongue
English
Weight
860 KB
Volume
6
Category
Article
ISSN
0006-3525

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✦ Synopsis

Hydrogen ion titration curves have been obtained for poly(riboadeny1ic acid) (poly A) at temperatures of 0-40Β°C. and ionic strengths of 0.001, 0.01, and 0.15. Where coniparable, the data are in general agreement with those previously reported by other investigators. Correlations between the titration data and thermal denaturation curves have been obtained. Formation of a two-stranded helix is catalyzed by the uptake of protons by the adenine base. Partial protonation of the base is required for formation of the two-stranded helix, but under appropriate conditions it is stable a t degrees of ionization less than 0.2. The degree of ionizatiou required for forniation of the two-stranded helix increases with temperature and decreases with ionic strength.

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