✦ LIBER ✦

Temperature dependence of sperm-egg fusion and post-fusion events in hamster fertilization

✍ Scribed by Hirao, Y. ;Yanagimachi, R.

Publisher: John Wiley and Sons
Year: 1978
Tongue: English
Weight: 391 KB
Volume: 205
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402050312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The effects of temperature (4–37°C) on sperm‐egg fusion and the post‐fusion events were studied. At 4–10°C, acrosome‐reacted spermatozoa bound to egg plasma membranes, but could not fuse with them. At 25°C or above, both binding and fusion took place. The post‐fusion events could occur over a broad temperature range (4–37°C) but the events progressed faster with increasing temperature. An abnormal development of egg pronucleus, possibly due to an incomplete functioning of the meiotic spindle mechanism, was observed in eggs inseminated at 37°C and cultured at 25°C.

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