Temperature dependence of proton–proton residual dipolar couplings in adenosine dissolved in charged phospholipid bicelles. Application of a two-dimensional selective refocusing method
✍ Scribed by Piotr Krajewski; Michel Bardet
- Book ID
- 102523920
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 335 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.1000
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✦ Synopsis
Abstract
^1^H NMR spectra of nucleoside adenosine were recorded in 7.5% (w/v) DMPC/DHPC bicelles doped with CTAB. Despite the small size of adenosine in comparison with the distances between discoidal bicelles, intramolecular ^1^H,^1^H residual dipolar couplings D were observed at 29.5–38°C. The absolute values of ^1^H,^1^H spin interactions Δ (sum of J‐ and D‐couplings) were measured selectively by a series of 2D SERF experiments as a function of temperature. At lower temperatures dipolar couplings are scaled down, which leads to simplification of the ^1^H NMR spectra. For some pairs of protons, which are simultaneously J‐ and D‐coupled, the exact values of D can be determined from temperature dependence of Δ. Copyright © 2002 John Wiley & Sons, Ltd.