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Temperature dependence of Na+−H+ exchange, Na+−HCO3− co-transport, intracellular buffering and intracellular pH in guinea-pig ventricular myocytes

✍ Scribed by Frederick F.-T. Ch'en; Emma Dilworth; Pawel Swietach; Ruth S. Goddard; Richard D. Vaughan-Jones


Book ID
110112465
Publisher
Cambridge University Press
Year
2003
Tongue
English
Weight
353 KB
Volume
552
Category
Article
ISSN
0022-3751

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Intracellular pH (pH i ) was measured at 37°C in mature rat cerebellar oligodendrocytes dissociated in culture by using the pH-sensitive probe BCECF. Cells were identified by anti-galactocerebroside antibody. The mean steady-state pH i was 7.02 in the absence of CO 2 /bicarbonate (Hepes-buffered sol