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Temperature dependence of CD spectra of DNA from various sources

✍ Scribed by A. F. Usatyi; L. S. Shlyakhtenko

Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
310 KB
Volume
12
Category
Article
ISSN
0006-3525

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✦ Synopsis

Synopsis

The CD spectra of DNA from various sources (T2; T4; Cd; Escherichia coli; calf thymus; Streptomyces chrysomalis) were investigated. A new band Asz10 in the CD spectra of glucosylated DNA of the T even phages was found. The temperature dependence of the CD spectra of DNA was obtained over a wide range of temperatures, including those of the helix-coil transition.

The band h 2 7 6 for all DNAs does not appreciably change in the range of the helixcoil transition. The monotonic increase of this band before melting, and its decrease after melting is observed with an increase in temperature.

The amplitude of the CD band h Z 4 5 for all the DNAs studied and Aezlo (glucosylated DNA) parallels the change of E ~w absorbance.

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