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Temperature and Quenching Studies of Fluorescence Polarization Detection of DNA Hybridization

✍ Scribed by Kumke, Michael U.; Shu, Luchuan; McGown, Linda B.; Walker, G. Terrance; Pitner, J. Bruce; Linn, C. Preston

Book ID
126234328
Publisher
American Chemical Society
Year
1997
Tongue
English
Weight
248 KB
Volume
69
Category
Article
ISSN
0003-2700

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The binding of proflavine to L)NA has been studied by measuring the polarization and intensity of emission of DNA-dye complexes. Such measurements also permit the determination of the fluorescence of the bound dye as a function of the degree of binding. Techniques of emission spectroscopy permit the