Temperature and hydrostatic pressure effects on gelatinase activity of a Vibrio sp. and partially purified gelatinase

✍ Scribed by Mary S. Weimer; Prof. Dr. R. Y. Morita

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 400 KB
• Volume: 14
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19740140810

✦ Synopsis

Abstract

The production of gelatinase by psychrophilic Vibrio sp. and its subsequent activity were shown to be maximal at 24°C (the highest growth temperature for the organism) and pH 8.8. Hydrostatic pressure caused a decrease in cells' gelatinase production and activity.

Gelatinase, partially purified by ammonium sulfate fractionation and column chromatography, demonstrated optimal activity at 40°C and pH 9.3. In the absence of NaCl, no activity was observed while optimal activity occurred at 4.0% NaCl concentration. A greater decrease in activity was caused by hydrostatic pressure at 25°C than at 40°C.