Telomere length measurement by fluorescence in situ hybridization and flow cytometry: Tips and pitfalls
β Scribed by Gabriela M. Baerlocher; Jennifer Mak; Teri Tien; Peter M. Lansdorp
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 275 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0196-4763
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Telomeres are G/C-rich repetitive DNA sequences at the end of all eukaryotic chromosomes. The loss of telomeric repeat sequences during cell divisions has been proposed as a possible mechanism for cell senescence. The standard procedure for measurement of telomere length is Southern blot (SB) hybrid
## Abstract Flow cytometry is a powerful tool for measuring parasitemias in murine malaria models used to test new antimalarials. Measurement of the emission of the nonpermeable nucleic acid dye YOYOβ1 (at 530 and 585 nm after excitation at 488 nm) allowed the unambiguous detection of low parasitem